Insights gained from single-cell RNA analysis of murine endothelial cells in aging hearts

Aging is the strongest risk factor for cardiovascular disease, with progressive decline in function of vascular endothelial cells (ECs) age. Systematic analyses effects aging on different cardiac EC types remain limited. Here, we constructed a scRNA atlas transcriptomes young and old mouse hearts. We identified 10 subclusters. The multidimensionally differential genes (DEGs) analysis across clusters shows molecular changes aging, showing increase overall inflammatory microenvironment decrease angiogenesis cytoskeletal support capacity aged ECs. And performed an in-depth 3 special ECs, Immunology, Proliferating Angiogenic. Immunology seems highly associated some immune regulatory functions, which at degrees. Analysis two neovascular Proliferating, Angiogenic, implied that Angiogenic ECs can differentiate into multiple directions after initially originating from proliferating leads to ability differentiation. Finally, summarized cell signaling communication between clusters. This offers comprehensive insights regulations provides new prevention treatment age-related disease.